Original scientific paper
Qualitative and quantitative analysis of salivary microorganisms in subjects with xerostomia
Ana Cekić-Arambašin
; Zavod za bolesti usta, Stomatološkog fakulteta Sveučilišta u Zagrebu, Zagreb, Hrvatska
Kristijan Temmer
; Zavod za bolesti usta, Stomatološkog fakulteta Sveučilišta u Zagrebu, Zagreb, Hrvatska
Zoran Bašić
; Zavod za mikrobiologiju, Stomatološki fakultet, Zagreb, Hrvatska
Abstract
Saliva, with its characteristic oontent of organic and anorganic substances making it antimicrobially active, is one of the basic protective mechanisms of the oral cavity. Therefore, the clinical oral symptom of inflammation is more frequently seen in subjects suffering from xerostomia. Our intention was, however, to investigate whether there were any differences in the amount and content of oral microorganisms in persons with verified xerestomia but free of any clinical signs of gingivitis, in comparison to the subjects with both normal amount of saliva and intact oral mucosa. For this purpose, 1 ml of saliva was collected by means of a sterile syringe. The 106 and 107 dilutions were prepared and 0.1 ml of it cultured on a nutritive blood agar. Following a 24-h incubation at 37 C, the grown colonies
were counted. Microorganisms were identified using the standard microbiological methods. The results obtained did not reveal any differences in the proportions of the kinds of microorganisms in the saliva from the groups under study. The differences observed in the quantity of microorganisms, which was substantially higher in the subjects with xerostomia, point to the possible important role of saliva in the oral cavity eubiosis and genesis of pathologic inflammatory alterations in the conditions of a reduced amount of saliva.
Keywords
Hrčak ID:
105147
URI
Publication date:
15.3.1986.
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