Original scientific paper
In vitro sensitivity of tooth germs to immune spleen cells in rats
Dragica Zaklan-Kavić
; Zavod za oralnu kirurgiju Stomatološkog fakulteta, Zagreb
I. Bašić
; Zavod za animalnu fiziologiju Prirodoslovno-matematičkog fakulteta, Zagreb
Abstract
In vitro citotoxicity of immune spleen cells to tooth germs is described in this study. Three-month-old inbred Y59 rats were immunized either with tooth germs or with skin graft and spleen cells originated from VM rats. Tooth germs from 10-days-old VM rats were incubated at 37°C in 10% CO2 atmosphere with spleen cells from immunized or nonimmunized animals. Following 24 or 96 hours incubation the tooth germs were transplanted under the kidney capsula of the syngeneic recipients. The recipients were killed from 7 to 35 days after transplantation, grafts removed and histologicly analised. The results showed that the soft tissue of the grafted tooth germs incubated with immunized spleen cells undergo necrosis. All grafts become acellular within 14 days after transplantation in syngeneic recipients and they did not recover until 35 days after transplantation. On the other hand, the tooth germs incubated with allogeneic or syngeneic spleen cells as well as those incubated with no presence of the lymphoid cels maintained their ability to recover, develop and differentiate in syngeneic recipient. This indicates that tooth germs induce cellular immune response in allogeneic host and that the spleen cells of these animals are capable of destroying soft tissue of the tooth germs when incubated in vitro.
Keywords
Hrčak ID:
105815
URI
Publication date:
15.3.1980.
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