Original scientific paper
The toxicity of chelator on cell elements in vitro
Dora Najžar-Fleger
; Zavod za dentalnu patologiju Stomatološkog fakulteta, Zagreb
S. Smerdel
; Odjel za rad na području virusa Imunološkog zavoda, Zagreb
Abstract
The cytotoxicity of chelator and NaEDTA with a, pH of 7,05 — (Central Pharmacy of the Clinical Hospitals, Ljubljana) on the linear culture of He-La cells in vitro was investigated. The minimal cytotoxic dose for the He-La cells culture was at first determined by means of chelator titration in various dilutions (50 000/ml) in EME which was found in a dilution of 1:61. Subsequently the role of Calcium and Magnesium ions was investigated on the course of disintegration of intercellular substances of the HeLa cells culture in the media with calcium and magnesium ions (EME) and without the ions mentioned (PBS) at equal pH values (pH 7,2). The results show that the disintegration of intercellular connections developed at the same pH in an identical manner regardless of the presence of calcium and magnesium in the nutritive medium. On the base of this finding the authors conclude that a small quantity of calcium and magnesium ions is necessary for the chelating activity and that in a medium without these ions the chelator extracts it from the intercellular substance and from the very cells. The authors moreover mention that these results might be important for the study of the chelator phenomenon on the tissue of the dental enamel because it shows that on account of great sensitivity of the tissue to chelator activity (1:61) disintegration of the interprismatic substance may rapidly occur with regard to the fact that the density of the enamel is not essential for the entire mechanism.
Keywords
Hrčak ID:
108121
URI
Publication date:
15.9.1971.
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