Veterinary Archives, Vol. 83 No. 6, 2013.
Original scientific paper
Eradication of bovine leukosis virus on a diary farm through improved virus detection.
Ivana Lojkić
orcid.org/0000-0003-1559-9020
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Davor Balić
; Veterinary Department Vinkovci, Croatian Veterinary Institute, Vinkovci, Croatia
Nevenka Rudan
; Faculty of Veterinary Medicine, University of Zagreb, Croatia
Mislav Kovačić
; Krka-farma, Zagreb d.o.o.
Željko Čač
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Marin Periškić
; Veterinary Department Vinkovci, Croatian Veterinary Institute, Vinkovci, Croatia
Tomislav Bedeković
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Besi Roić
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Irena Ciglar Grozdanić
; Faculty of Veterinary Medicine, University of Zagreb, Croatia
Abstract
The aim of this study was to perform a diagnosis and molecular characterisation of bovine leukosis virus (BLV) on a Croatian dairy farm. For that purpose we compared the diagnostic tools used in our study, made sequence and phylogenetic analysis of our BLV and tried to describe the difficulties in the process of EBL eradication on the examined farm after reintegration into Croatian territory after the war. From 1998 to 2008 blood samples from a dairy farm in the North-eastern part of Croatia were tested serologically using AGID and ELISA. In 2002, 2003 and 2004 37%, 22% and 10% of animals were serologically positive, respectively. After the initial eradication steps, the disease reappeared in 2008, when all examined blood samples reacted positively in BLV-specific nested PCR. Finally, at the end of 2010, after an extended eradication program, which included the implementation of PCR together with regular ELISA testing for detection of positive animals, the farm obtained the status of “free of BLV”. Sequence and phylogenetic analysis of 524-nt fragment of BLV env gp51 encoding gene showed the greatest identity with the Croatian BLV genotype 8 isolates, so it was phylogenetically clustered with those isolates.
Keywords
bovine leukosis virus; gene env; gp51; ELISA; nested PCR; phylogenetic analysis
Hrčak ID:
111798
URI
Publication date:
9.12.2013.
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