Acta Pharmaceutica, Vol. 64 No. 1, 2014.
Original scientific paper
https://doi.org/10.2478/acph-2014-0006
The effect of diclofenac on proliferation and production of growth factors by endothelial cells (hmec-1) under hypoxia and inflammatory conditions
ANNA WIKTOROWSKA-OWCZAREK
; Department of Pharmacology, Chair of Pharmacology and Clinical Pharmacology, Medical University of Lodz, Poland
Abstract
Diclofenac belongs to non-steroidal anti-inflammatory drugs (NSAIDs) and non-selective COX inhibitors. The aim of this study was to examine the effect of diclofenac on endothelial cells proliferation under the influence of hypoxia or inflammatory conditions. Another goal was to check whether diclofenac modulates the secretion of angiogenic factors such as VEGF and bFGF in human microvascular endothelial cells (HMEC-1) in the presence of CoCl2 or lipopolysaccharide (LPS), which could influence the endothelial cells in an autocrine manner or other cells in a paracrine manner. HMEC-1 cells were treated with 0.1 and 0.3 mmol L-1diclofenac in the presence of 100 μgmL-1 LPS or 200 μmol L-1CoCl2. Diclofenac decreased cell viability under hypoxia and inflammatory conditions. The stimulation of bFGF secretion by LPS in microvascular endothelial cells (HMEC-1 cell) was attenuated bydiclofenac. Diclofenac increased the secretion of VEGF induced by LPS and hypoxia.
Keywords
LPS; VEGF; bFGF; diclofenac; hypoxia; endothelial cells
Hrčak ID:
113588
URI
Publication date:
31.3.2014.
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