Preliminary communication

Methods of Preparing the Tooth for DNA Isolation

Željka Presečki
Hrvoje Brkić
Dragan Primorac
Irena Drmić

Abstract

In this paper, the importance of the tooth in identification an unknown person has been demonstrated.
The theoretical part shows the difference between genomic and mitochondrial DNA. That difference is the durability and properties which they carry and the value they have in identification. Microanatomy has also been described, possible locations of cells in
the tooth and methods to collect material for DNA extraction.
The practical part shows the methods used in particular cases for DNA isolation. Horizontal section through the cervical root is used to collect material for DNA isolation. Genomic DNA , from the material, is extracted by organic phenol-chloroform methods. The results are shown on 1 % agarose gel and photographed by a Polaroid camera.

Keywords

DNA, tooth; identification; forensic dentistry; AMEL gene; DNA isolation; phenol-chloroform organic method; horizontal section

Hrčak ID:

11035

URI

https://hrcak.srce.hr/11035

Publication date:

15.3.2000.

Article data in other languages: croatian

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