Original scientific paper

https://doi.org/10.2478/acph-2014-0020

Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters

PRZEMYSLAW M. MROZIKIEWICZ ; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 61-861 Poznan, Poland; Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres a
ANNA BOGACZ ; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 61-861 Poznan, Poland; Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres a
JOANNA BARTKOWIAK-WIECZOREK ; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 61-861 Poznan, Poland; Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres a
RADOSLAW KUJAWSKI ; Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, 61-707 Poznan, Poland
PRZEMYSLAW L. MIKOLAJCZAK ; Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, 61-707 Poznan, Poland; Department of Pharmacology, University of Medical Sciences, 60-806 Poznan, Poland
MARCIN OZAROWSKI ; Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, 61-707 Poznan, Poland; Departament of Pharmaceutical Botany and Plant Biotechnology, University of Medical Sciences, 61-861 Pozn
BOGUSLAW CZERNY ; Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, 61-707 Poznan, Poland; Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, 70-204 Szczecin,
BEATA MROZIKIEWICZ-RAKOWSKA ; Department of Gastroenterology and Metabolic Diseases, Medical University of Warsaw, 02-091 Warsaw, Poland
EDMUND GRZESKOWIAK ; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 61-861 Poznan, Poland

Abstract

There are a number of compounds that can modify the activity of ATP-binding cassette (ABC) and solute carrier (SLC) transporters in the blood-brain barrier (BBB). The aim of this study was to investigate the effect of natural and synthetic substances on the expression level of genes encoding transporters present in the BBB (mdr1a, mdr1b, mrp1, mrp2, oatp1a4, oatp1a5 and oatp1c1). Our results showed that verapamil caused the greatest reduction in the mRNA level while other synthetic (piracetam, phenobarbital) and natural (codeine, cyclosporine A, quercetin) substances showed a selective inhibitory effect. Moreover, extract from roots of Panax ginseng C. A. Meyer exhibited a decrease of transcription against selected transporters whereas extract from Ginkgo biloba L. leaves resulted in an increase of the expression level of tested genes except for mrp2. Extract from aerial parts of Hypericum perforatum L. was the only one to cause an increased mRNA level for mdr1 and oatp1c1. These findings suggest that herbs can play an important role in overcoming the BBB and multidrug resistance against pharmacotherapy of brain cancer and mental disorders, based on the activity of selected drug-metabolizing enzymes and transporters located in the BBB.

Keywords

ABC proteins; P-glycoprotein; blood-brain barrier; expression level; herbal substances

Hrčak ID:

114604

URI

https://hrcak.srce.hr/114604

Publication date:

30.6.2014.

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