Original scientific paper
https://doi.org/10.3325/cmj.2014.55.19
Reliability of long vs short COI markers in identification of forensically important flies
Sanaa M. Aly
; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Suez Canal University Ismailia, Egypt
Abstract
Aim To compare the reliability of short and long cytochrome
oxidase I gene fragment (COI) in identification of
forensically important Diptera from Egypt and China.
Methods We analyzed 50 specimens belonging to 18 species.
The two investigated markers were amplified by polymerase
chain reaction (PCR) followed by direct sequencing.
Nucleotide sequence divergences were calculated
using the Kimura two-parameter (K2P) distance model and
neighbor-joining (NJ) phylogenetic trees.
Results Although both tested fragments showed an overlap
between intra and interspecific variations, long marker
had greater completeness of monophyletic separation
with high bootstrap support. Moreover, NJ tree based on
the long fragment clustered species more in accordance
with their taxonomic classification than that based on the
short fragment.
Conclusion In dipterous identification, it is recommended
to use the long COI marker due to its greater reliability and
safety.
Keywords
Hrčak ID:
117032
URI
Publication date:
15.2.2014.
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