Original scientific paper
Antibacterial Activity of Calcium Hydroxide Root Canal Sealer (Apexit) - in vitro Study
Sonja Pezelj-Ribarić
Ivana Brekalo
Ivana Miletić
Maja Abram
Zoran Karlović
Ivica Anić
Abstract
The aim of this study was to determine the in vitro antimicrobial activity of calcium hydroxide root canal sealer (Apexit) in direct contact with Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens and Staphylococcus aureus.
The direct contact test (DCT) was performed. The sealer was mixed and placed on the side wall of microtiter plate wells and 10 μl of bacterial suspension (106 bacteria) was placed onto its surface. Bacteria were in direct contact with the sealer for 1 hour at 37°C. BHI broth (250 μl) was then added and the growth of each strain was measured after 1 hour, 6 hours, 20 hours and 24 hours.
After 1 hour the number of E. coli and P. aeruginosa decreased to 7-9 x 10 3 and S. marcescens to 7 x 10 2 bacteria. The number of S. aureus was 1.4 x 10 5 bacteria. Six hour samples showed that the number of P. aeruginosa decreased to 10 1 and gram-positive S. aureus to 7.5 x 10 4. After 20 hours only S. aureus (10 1 bacteria) survived after prior contact with Apexit.
The 24-hour samples showed complete bacterial growth inhibition of all the bacterial strains tested in DCT with Apexit.
Keywords
antibacterial effect; Direct contact test; Apexit
Hrčak ID:
11427
URI
Publication date:
15.12.2001.
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