Original scientific paper
Cytotoxicity of AH Plus and AH26 in vitro on Chinese hamster V79 fibroblasts
Ivana Miletić
Silvana Jukić
Ivica Anić
Zoran Karlović
Sonja Pezelj-Ribarić
Maja Osmak
Abstract
The purpose of the study was to evaluate cytotoxicity of AH Plus, compared to AH26 in vitro on Chinese hamsters V79 fibroblasts. The materials were prepared according to the manufacturer's instructions. After the initial setting period, the materials were crumbled and dissolved in dimetil-sulfoxide solution. The extracts obtained were incubated at 37C during one hour, 24 hours and 7 days. After incubation period the samples were diluted with Eagle’s minimum essential medium to concentrations ranging from 1.67 μg/ml to 167 μg/ml. Each concentration was placed on a plate with 24 wells with 5x103 V79 cells per 1.2 ml of medium. The number of cells was counted by electronic counter and the percentage of viable cells was determined by a light microscope. Both materials, AH26 and AH Plus were found to be cytotoxic. In both materials cytotoxic effect was related to the concentration of endodontic sealer in the extract solution. The critical concentrations, above which the sealers totally destroy the cell line are between 5.57 μg/ml and 167 μg/ml for AH Plus and 16.57 μg/ml and 167 μg/ml for AH26. The setting time did not have a statistically significant effect an cytotoxicity.
Keywords
chinese hamster V79 fibroblasts; AH Plus; AH26; cytotoxicity
Hrčak ID:
11444
URI
Publication date:
15.9.2000.
Visits: 3.171 *