Original scientific paper

E-Selectin expression in the mouse testis after experimental testicular torsion (ischemia/reperfusion).

Murat Selcuk ; Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Turkey
Muzaffer Celebi ; Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Turkey
Mustafa Y. Gulbahar ; Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Pathology, Turkey
Yonca B. Kabak ; Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Pathology, Turkey
Mesut Cevik ; Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Turkey

Abstract

Germ cell-specific apoptosis occurs after ischemia/reperfusion of the testis and is dependent on E-selectin expression. The aim of the study was to determine differences in E-selectin expression in testes tissues of control, sham and treatment groups after ischemia/reperfusion in mice. Mice were subjected to 720° testicular torsion for 1 h or 2 h duration (ischemia) followed by detorsion (reperfusion). After 2 h of reperfusion, the testes were fixed in Bouin fixative and immunohistochemical analysis performed for E-Selectin expression. E-selectin expression increased in the ischemic testis and contralateral testis after 2 h of reperfusion in mice. This increase in E-selectin expression may confirm that E-selectins play a key role in mediating of apoptosis in germ cells after ischemia/reperfusion. Thus, the blockage of E-selectins might be a strategy for rescue of post-ischemic testes.

Keywords

E-selectin; ischemia; reperfusion; mouse; testis

Hrčak ID:

119044

URI

https://hrcak.srce.hr/119044

Publication date:

31.3.2014.

Article data in other languages: croatian

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