Original scientific paper

Effect of duration of diabetes mellitus type 1 in children and adolescents on urinary N-acetyl-β-D-glucosaminidase excretion

Jasminka Matica
Višnja Rački-Čargonja
Štefica Dvornik

Abstract

Objective: The aim of this study was to examine whether there are differences between urinary NAG activity between children and adolescents with diabetes mellitus type 1 and healthy subjects and examine whether urinary NAG activity changes in relation to duration of the disease.
Methods: N-acetyl-β-D-glucosaminidase (NAG) activities were determined spectrophotometrically in random urine samples children and adolescents (N=66) with type 1 diabetes mellitus and control subjects (N=68). Patients in the diabetic group were divided according to the duration of diabetes in four groups: group I, less than three years (N=17); group II, three to five years (N=19); group III, five to ten years (N=19) and group IV, more than ten years (N=11). To exclude interference due to urine concentration differences, urinary NAG activities were referred to the level of urinary creatinine.
Results: Urinary NAG excretion in diabetic patients was significantly increased compared to the controls (P<0.001). All four subgroups had significantly higher activities of urinary NAG compared to the controls (group I, P=0.001, groups II and III P< 0.001 and group IV, P=0.004) butthere was no significant difference among diabetic groups (group I vs. II, P=0.716; group I vs. III, P=0.899; group I vs. IV, P>0.10; group II vs. III, P=0.549; group II vs. IV, P>0.10 and group III vs. IV, P>0.10). There was no correlation between urinary NAG activities in diabetic patients and duration of diabetes (r= - 0.017, P=0.892).
Conclusion: Urinary NAG excretion was elevated in children and adolescents with diabetes mellitus type I but it did not depend on duration of the disease.

Keywords

N-acetyl-β-D-glucosaminidase; diabetes mellitus type 1; diabetic nephropathy

Hrčak ID:

11535

URI

https://hrcak.srce.hr/11535

Publication date:

15.5.2006.

Article data in other languages: croatian

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