Original scientific paper
Association Study of Cytochrome P450 1A1*2A Polymorphism with Prostate Cancer Risk and Aggressiveness in Croatians
Sanja Mandić
; »J. J. Strossmayer« University, University Hospital Centre Osijek, Department of Clinical Laboratory Diagnostics, Osijek, Croatia
Vesna Horvat
; »J. J. Strossmayer« University, University Hospital Centre Osijek, Department of Clinical Laboratory Diagnostics, Osijek, Croatia
Saška Marczi
; »J. J. Strossmayer« University, University Hospital Centre Osijek, Clinical Institute of Nuclear Medicine and Radiation Protection, Department of Molecular Diagnostics and Tissue Typing Osijek, Croatia
Ivana Lukić
; »J. J. Strossmayer« University, School of Medicine, Osijek, Croatia
Josip Galić
; »J. J. Strossmayer« University, University Hospital Centre Osijek, Department of Urology, Osijek, Croatia
Abstract
Cytochrome P450 1A1 (CYP1A1) is an enzyme participating in the bioactivation of various endogenous and environmental reactive compounds that can bind to DNA and thus induce cancerogenesis. Gene encoding the enzyme is expressed in the prostate tissue and is polymorphic. CYP1A1*2A gene polymorphism is associated with elevated enzyme activity and/or inducibility which can lead to accumulation of genotoxic compounds and consequently to cancerogenesis. We examined the association of this polymorphism with prostate cancer (PCa) risk and aggressiveness. The case-control study consisted of 120 PCa patients and 120 benign prostatic hyperplasia (BPH) controls, in Croatian population. Regarding aggressiveness, PCa patients were grouped according to the Gleason score (GS), tumor stage (T) and existence of distant metastasis (M). The polymorphism was analyzed using real-time polymerase chain reaction (PCR). We did not observe association of mutated allele with PCa risk, neither with PCa aggressiveness. Furthermore, frequency of polymorphic genotype was slightly higher in BPH group (16.6% vs. 14.2%, respectively) and also in less aggressive form of PCa (20.4% vs. 9.6% for GS<7; 15.6% vs. 9.1% for T<3; 16.7% vs. 10.0% for no distant metastasis). Comparing our findings with other published results, we can assume that the ethnicity influence the genotype distribution and thus may affect the etiology of PCa, even possibly in the way to cause an opposite effect among different ethnic groups. Given the small number of participants, results should be validated on the larger sample size.
Keywords
CYPA1A1; single nucleotide polymorphism; prostate cancer; Croatia; polycyclic aromatic hydrocarbons; smoking; DNA adducts; frequency; Gleason score; metastasis
Hrčak ID:
120865
URI
Publication date:
31.3.2014.
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