Original scientific paper

Isolation, molecular characterization and antibiotic resistance of Enterotoxigenic E. coli (ETEC) and Necrotoxigenic E. coli (NTEC) from healthy water buffalo.

Achintya Mahanti ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences 37, K. B. Sarani, Kolkata, West Bengal, India
Indranil Samanta orcid.org/0000-0003-1189-3994 ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences 37, K. B. Sarani, Kolkata, West Bengal, India
Samiran Bandyopadhyay orcid.org/0000-0002-9529-5715 ; Eastern Regional Station, Indian Veterinary Research Institute, West Bengal, India
Siddhartha N. Joardar ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences 37, K. B. Sarani, Kolkata, West Bengal, India
Tapan K. Dutta ; Department of Veterinary Microbiology, Central Agricultural University, Aizawl Mizoram, India
Tapas K. Sar ; Department of Veterinary Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences 37, K. B. Sarani, Kolkata, West Bengal, India

Abstract

The present study was undertaken to detect the prevalence, virulence gene profile and antibiotic resistance of Enterotoxigenic Escherichia coli (ETEC) and Necrotoxigenic E. coli (NTEC) in healthy water buffalo in West Bengal. Out of the 363 E. coli isolates from 165 faecal samples, 18 (4.95%) and 7 (1.9%) isolates were found to possess genes for ETEC (LT or STa or STb) and NTEC (cnf1 or cnf2), respectively in PCR. Among the 18 ETEC isolates, 13 (72.22%), 12 (66.67%), and 1 (5.56%) isolates were found to harbour STa, LT, and STb genes respectively. However, among the 7 NTEC isolates, 3 (42.85%) and 4 (57.14%) were detected to possess cnf1 and cnf2 genes, respectively. Further, among the five isolates possessing the EAST 1 gene, four were detected with the STa gene and one (1) with the LT gene. However, the majority of the F41 strains possessed ST genes, and F5 strains harboured both LT and ST. In addition, one out of three cnf1 NTEC isolates was detected to harbour the papC gene and all the four cnf2 NTEC isolates contained the cdt gene. The ETEC isolates belonged to 11 different serogroups (O11, O53, O71, O84, O88, O103, O112, O120, O128, O153, and O158), while, four NTEC isolates belonged to the OUT serogroup and the remaining 3 were disseminated among the O2, O4, and O6 serogroups. The RAPD of the ETEC isolates produced different electrophoretic profifi les. The antibiotic resistance of ETEC and NTEC isolates was observed most frequently to amikacin (56%), kanamycin (44%), gentamicin (40%), neomycin (36%). The study thus revealed water buffalo as a reservoir of multi drug resistant ETEC/NTEC for the first time in India.

Keywords

antibiotic resistance; water buffalo; E. coli; India; enterotoxigenic; necrotoxigenic; polymerase chain reaction

Hrčak ID:

122012

URI

https://hrcak.srce.hr/122012

Publication date:

28.5.2014.

Article data in other languages: croatian

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