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Veterinary Archives, Vol. 84 No. 3, 2014.

Original scientific paper

Application of seminested PCR for detection of larval stages of Spirocerca lupi in garden lizards (Calotes versicolor).

Reghu Ravindran orcid id orcid.org/0000-0001-9250-0552 ; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India
Malayil Aparna ; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India
Joju Johns ; Department of Veterinary Surgery, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India
Ullisseri C. Mithin ; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India
Sincy Varghese ; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India
Gopi Jyothimol ; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India
Leena Chandrasekhar ; Department of Veterinary Anatomy, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India
Karapparambu G. AjithKumar ; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad, Kerala, India

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Abstract

The study reveals the usefulness of semi-nested PCR as a tool to identify larval stages of Spirocerca lupi in garden lizards (Calotes versicolor), the paratenic host for the parasite. Creamy white cysts present in the superficial muscles of seven out of thirteen garden lizards on the veterinary college campus, Pookode, were recovered and observed for the presence of live larvae. DNA isolated from these cysts and S. lupi worms recovered during postmortem examination of an infected dog were used for semi-nested PCR for amplifying an internal region of the S. lupi Cox-I gene, producing a specific 400 bp fragment. Thus the semi-nested PCR may be utilized for specific detection of larval stages of S. lupi in a paratenic host.

Keywords

canine spirocercosis; Spirocerca lupi; garden lizards; semi-nested PCR

Hrčak ID:

122236

URI

https://hrcak.srce.hr/122236

Publication date:

28.5.2014.

Article data in other languages: croatian

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