Original scientific paper

https://doi.org/10.5562/cca2272

Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity

Mirna Ćurković-Perica ; Department of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9a, 10000 Zagreb, Croatia
Saša Likić ; Department of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9a, 10000 Zagreb, Croatia
Gordana Rusak ; Department of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9a, 10000 Zagreb, Croatia

Abstract

Quantitative and qualitative analysis of phenolic compounds in tissue extracts from Centaurea rupestris L. as well as their antiphytoviral activity against Tomato bushy stunt virus was performed.
Extracts of flowers, leaves and roots from C. rupestris growing in the wild, as well as extracts from shoots, tissue consisting of callus and shoots and undifferentiated callus grown in vitro, were tested.
Between tested extracts predominantly quantitative and only several qualitative differences in phenolics were detected by high performance liquid chromatography. The highest amounts of quercetagetin
3’-methylether-7-O-ß-D-glucopyranoside and quercetin were detected in flowers while in leaves the high-est amounts of luteolin, caffeic and p-coumaric acid were detected. Except for roots, antiphytoviral activi-ty of all other extracts was high, inducing virus inhibition, ranging from 43 to 90 %. Simultaneous appli-cation of quercetin, caffeic or p-coumaric acid with virus decreased the number of lesions indicating that these substances contribute to the antiphytoviral activity of C. rupestris extracts. (doi: 10.5562/cca2272)

Keywords

Asteraceae; extracts; flavonoids; HPLC; tissue culture; Tomato bushy stunt virus

Hrčak ID:

122283

URI

https://hrcak.srce.hr/122283

Publication date:

30.4.2014.

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