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Archives of Industrial Hygiene and Toxicology, Vol. 65 No. 2, 2014.

Original scientific paper

https://doi.org/10.2478/10004-1254-65-2014-2392

Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro

Elizabeta Galić ; Health Center of Split-Dalmatia County, Split, Croatia
Antonija Tadin orcid id orcid.org/0000-0002-5365-9816 ; Study of Dental Medicine, School of Medicine, University of Split, Slit, Croatia
Nada Galić ; Department of Endodontics and Restorative Dental Medicine, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Vilena Kašuba ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Marin Mladinić ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ružica Rozgaj ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Dolores Biočina-Lukenda ; Study of Dental Medicine, School of Medicine, University of Split, Slit, Croatia
Ivan Galić ; Study of Dental Medicine, School of Medicine, University of Split, Slit, Croatia
Davor Želježić ; Institute for Medical Research and Occupational Health, Zagreb, Croatia

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Abstract

The purpose of this study was to evaluate the genotoxic potential of components leached from two conventional self-curing glass-ionomer cements (Fuji IX and Ketac Molar), and light-curing, resin modified glass-ionomer cements (Vitrebond, Fuji II LC). Evaluation was performed on human lymphocytes using alkaline and hOGG1 modified comet, and micronucleus assays. Each material, polymerised and unpolymerised, was eluted in extracellular saline (1 cm2 mL-1) for 1 h, 1 day, and 5 days. Cultures were treated with eluates using final dilutions of 10-2, 10-3, and 10-4. Alkaline comet assay did not detect changes in DNA migration of treated cells regardless of the ionomer tested, polymerisation state, and elution duration. Glass ionomers failed to significantly influence micronucleus frequency. No oxidative DNA damage in treated lymphocytes was observed using hOGG1 modified comet assay. Obtained results indicate high biocompatibility of all tested materials used in the study under experimental conditions.

Keywords

biocompatibility; cytotoxicity; DNA damage; genotoxicity; lymphocytes

Hrčak ID:

122939

URI

https://hrcak.srce.hr/122939

Publication date:

11.6.2014.

Article data in other languages: croatian

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