Short communication, Note

https://doi.org/10.2478/acph-2014-0033

Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves

MARCOS SALAZAR ; Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET), Facultad de Medicina, Universidad de Panamá; Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá
LILIA CHÉRIGO ; Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá
HILDAURA ACOSTA ; Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET), Facultad de Medicina, Universidad de Panamá
RAFAEL OTERO ; Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia
SERGIO MARTÍNEZ-LUIS ; Center for Biodiversity and Drug Discovery, INDICASAT-AIP, Panama, Panama

Abstract

Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg extract kg–1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 16.1 µg of venom kg–1 b.m.) lowered the hemorrhagic activity of the venom in CD-1 mice by 51.5 ± 2.6 %. Additionally, 1.7 mg extract L–1 plasma prolonged 5.1 times the plasma coagulation time. Fractionation of the extract led to isolation of two compounds: ononitol (1) and quercetrin (2). The structure of compounds 1 and 2 was established by spectroscopic analyses, including APCI-HR-MS and NMR (1H, 13C, HSQC, HMBC and COSY). Quercetrin prolonged 2.6 times the plasma coagulation time demonstrating that this compound was one of the active constituents of the Brownea rosademonte extract.

Keywords

Bothropsasper venom; Brownearosademonte; quercetrin; anticoagulant; antihemorrhagic; antiproteolytic; antihemolytic

Hrčak ID:

123211

URI

https://hrcak.srce.hr/123211

Publication date:

31.12.2014.

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