Original scientific paper
https://doi.org/10.15644/asc48/2.116
Detection of Sulphate-Reducing Bacteria and Others Cultivable Facultative Bacteria in Dental Tissues
Fabiano Luiz Heggendorn
; Department of Pathology, Medical School, Fluminense Federal University, Rio de Janeiro, Brazil
Lúcio de Souza Gonçalves
; Dental School, Estácio de Sá University, Rio de Janeiro, Brazil
Eliane Pedra Dias
; Department of Pathology, Medical School, Fluminense Federal University, Rio de Janeiro, Brazil
Christiane Heggendorn
; Stomatologist, Fluminense Federal University, Rio de Janeiro, Brazil
Márcia T. S. Lutterbach
; Laboratory of Biocorrosion and Biodegradation, National Institute of Technology, Brazil
Abstract
Aim: To detect for the presence of sulphate-reducing bacteria (SRB) and evaluate the possible association between SRB and cultivable facultative bacterial of oral sites with different periodontal conditions. Methods: The study was carried out on 9 samples from different oral sites in 8 patients (two samples were collected from the same patient). Material was collected using modified Postgate E culture medium, indicated for the growth and isolation of SRB. In addition, a reducing solution for anaerobic bacteria was used as a transport solution for facultative bacteria and identified by polymerase chain reaction amplification (PCR) and sequencing of the 16S rRNA gene. Results: SRB was found in 3 patient samples: the first in a root fragment, the second in a root fragment and a healthy tooth with vertical bone loss and a mobility degree of 3; and the third in a healthy tooth extracted for orthodontic treatment. In the final patient, the cultivable facultative species Lactobacillus casei was identified. Other facultative bacterial species were identified in patient 5 (Kurthia Gibsonii) and patient 7 (Pseudomonas aeruginosa). Conclusions: The detection of SRB in different dental tissues with distinct periodontal features demonstrated that new studies need to be developed in order to determine the true role of SRB in the oral microbiota. In addition, it was possible to verify the presence of Lactobacillus casei together with SRB in one sample.
Keywords
Periodontitis; Sulphur-Reducing Bacteria, Desulfovibrio
Hrčak ID:
124282
URI
Publication date:
1.7.2014.
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