Original scientific paper

Isolation, identification and molecular characterization of Pasteurella multocida isolates obtained from emu (Dromaius novaehollandiae) in Gujarat state, India.

Mayur P. Bhimani ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Ashish Roy ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Bharat B. Bhanderi ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Rafiyudin A. Mathakiya ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

Abstract

A total of 168 samples (bone marrow, lung tissue, liver and blood clot) were collected from 42 dead emus suspected to have died from fowl cholera. Samples were subjected for cultural isolation on blood agar. Of these, a total of 22 isolates of Pasteurella spp. were isolated and characterized biochemically and identified as P. multocida by PCR. Isolates were later tested for capsular type by multiplex PCR assay and all were found to be of capsular type A. This is the first report on the isolation, identification and molecular characterization of P. multocida from emu in the state of Gujarat, India. This study has revealed that emus are also susceptible to P. multocida (fowl cholera) and can act as a potential carrier of the organism.

Keywords

Pasteurella multocida; emu; PCR; capsular typing; fowlcholera

Hrčak ID:

125155

URI

https://hrcak.srce.hr/125155

Publication date:

16.7.2014.

Article data in other languages: croatian

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