Original scientific paper

https://doi.org/10.11613/BM.2014.030

Paraoxonase 1 activity and phenotype distribution in premenopausal and postmenopausal women

Dražan Butorac ; Clinic of Gynecology and Obstetrics, Medical School University Hospital Sestre Milosrdnice, Zagreb, Croatia
Ivana Ćelap ; University Department of Chemistry, Medical School University Hospital Sestre Milosrdnice, Zagreb, Croatia
Sanja Kačkov ; Medical biochemistry laboratory, Polyclinic Bonifarm, Zagreb, Croatia
Vera Robić ; Medical biochemistry laboratory, Polyclinic Aviva, Zagreb, Croatia
Tomislav Miletić ; Medical biochemistry laboratory, Polyclinic Aviva, Zagreb, Croatia
Zlata Flegar Meštrić Flegar Meštrić ; Department of Clinical Chemistry, Merkur University Hospital, Zagreb, Croatia
Andrea Hulina ; Department of Medical Biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Krunoslav Kuna ; Clinic of Gynecology and Obstetrics, Medical School University Hospital Sestre Milosrdnice, Zagreb, Croatia
Tihana Žanić Grubišić ; Department of Medical Biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Marija Grdić Rajković ; Department of Medical Biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia

Abstract

Introduction: Postmenopausal women have higher risk of cardiovascular disease. One of the contributing factors could be reduced activity of antiatherogenic enzyme paraoxonase 1 (PON1). The aim of this study was to examine differences in the lipid status, paraoxonase and arylesterase PON1 activities and PON1 phenotype in women with regular menstrual cycle and in postmenopausal women.
Materials and methods: The study included 51 women in reproductive age (25 in follicular and 26 in luteal phase of the menstrual cycle) and 23 women in postmenopause. Lipid parameters in sera were determined using original reagents and according to manufacturer protocol. PON1 activity in serum was assessed by spectrophotometric method with substrates: paraoxon and phenylacetate. PON1 phenotype was determined by double substrate method.
Results: Compared to the women in follicular and luteal phase, postmenopausal women have significantly higher concentration of triglyceride [0.9 (0.7-1.3), 0.7 (0.6-1.0) vs. 1.5 (0.9-1.7) mmol/L; P = 0.002], cholesterol [5.10 (4.78-6.10), 5.05 (4.70-5.40) vs. 6.30 (5.73-7.23) mmol/L; P < 0.001], LDL [3.00 (2.56-3.63), 3.00 (2.70-3.70) vs. 3.90 (3.23-4.50) mmol/L; P < 0.001], and apolipoprotein B [0.88 (0.75-1.00), 0.79 (0.68-1.00) vs. 1.07 (0.90-1.24) mmol/L; P = 0.002]. PON1 basal [104 (66-260), 106 (63-250) vs. 93 (71-165) U/L; P = 0.847] and salt-stimulated paraoxonase activity [210 (131-462), 211 (120-442) vs. 180 (139-296) U/L; P = 0.857] as well as arylesterase activity [74 (63-82), 70 (54-91) vs. 70 (60-81) kU/L; P = 0.906] and PON1 phenotype (P = 0.810) were not different in the study groups.
Conclusion: There are no differences in PON1 activity and PON1 phenotype between women with regular menstrual cycle and postmenopausal women.

Keywords

paraoxonase 1; postmenopausal women; phenotype; lipids

Hrčak ID:

125433

URI

https://hrcak.srce.hr/125433

Publication date:

15.6.2014.

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