Short communication, Note

https://doi.org/10.2478/v10007-007-0030-8

Assay of flavonoid aglycones from species of the genus Sideritis (Lamiaceae) from Macedonia with HPLC UV DAD

BISERA JANESKA
MARINA STEFOVA
KALINA ALIPIEVA

Abstract

Flavonoids obtained from Sideritis species (Lamiaceae), S. raeseri and S. scardica, grown in Macedonia were studied. Qualitative and quantitative analyses of the flavonoid aglycones were performed using high-performance liquid chromatography (HPLC) with a UV diode array detector. Extracts were prepared by acid hydrolysis in acetone, reextraction in ethyl acetate and evaporation to dryness; the residue dissolved in methanol was subjected to HPLC analysis.
Isoscutellarein, chryseriol and apigenin were identified in the extracts. Also, a 4’-methyl ether derivative of isoscutellarein was found, together with hypolaetin and its methyl ether derivative, which were identified according to previously isolated glycosides and literature data. Quantitation was performed using calibration with apigenin.
According to this screening analysis, the samples of the genus Sideritis from Macedonia are rich in polyhydroxy flavones and analogous with the previously studied Mediterranean Sideritis species from the Ibero-North African and Greek Sideritis species with respect to the presence of 8-OH flavones and their derivatives.

Keywords

flavonoids; polyhydroxy flavones; Sideritis; HPLC-UV DAD

Hrčak ID:

12063

URI

https://hrcak.srce.hr/12063

Publication date:

1.9.2007.

Article data in other languages: croatian

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