Original scientific paper

Apoptotic markers (P53, Bcl-2 and Bax) expression in renal oncocytoma and chromophobe renal cell carcinoma

ALMA DEMIROVIĆ ; University Department of Pathology Sestre milosrdnice University Hospital Centre, Zagreb, Croatia
MONIKA ULAMEC ; 1_University Department of Pathology Sestre milosrdnice University Hospital Centre, Zagreb, Croatia; 2_School of Medicine, University of Zagreb, Zagreb, Croatia
MIRAN BEZJAK ; School of Medicine, University of Zagreb, Zagreb, Croatia
MLADEN BELICZA ; Academy of Medical Sciences of Croatia, Zagreb, Croatia
BOŽO KRUŠLIN ; 1_University Department of Pathology Sestre milosrdnice University Hospital Centre, Zagreb, Croatia; 2_School of Medicine, University of Zagreb, Zagreb, Croatia

Abstract

Background and Purpose: Renal oncocytoma (RO) and eosinophilic
variant of chromophobe renal cell carcinoma (ChRCC) have overlapping
morphologic, immunohistochemical, histochemical and ultrastructural features. Their distinction is mandatory since RO is a benign tumor, while ChRCC is a low-grade malignant tumor which has metastatic potential and may undergo sarcomatoid transformation. The aim of this study was to determine expression of P53, Bcl-2 and Bax in ROs and ChRCCs and to explore whether these markers could be useful in differential diagnosis of RO and ChRCC.

Patients and Methods: We analyzed 61 cases (28 ChRCCs and 33
ROs) by immunohistochemistry using primary antibodies to P53, Bcl-2 and Bax. The staining percentage and staining intensity scores were multiplied to give immunohistochemical staining index (ISI).

Results and Conclusion: All specimens showed positive reaction for
Bcl-2 and Bax. There was no significant difference in the ISI for Bcl-2 and P53. Statistical analysis showed significant difference in the ISI of Bax between ROs and ChRCCs. Moreover, cases of ROs had cytoplasmic pattern of reaction for Bax, while the pattern of reaction for Bax in cases of ChRCCs was membranous. Further studies are needed to confirm the possible use of Bax immunostaining
in differential diagnosis of RO and ChRCC.

Keywords

Hrčak ID:

126357

URI

https://hrcak.srce.hr/126357

Publication date:

31.7.2014.

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