Original scientific paper

https://doi.org/10.5562/cca2241

Computer Assisted Method Development in Liquid Chromatography

Tomislav Bolanča ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia
Šime Ukić ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia
Mirjana Novak ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia
Marko Rogošić ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia

Abstract

This paper describes potential applications of computer-assisted chemometrics in method devel-opment in liquid chromatography. These include modeling of retention (isocratic, gradient, molecular modeling, artificial neural networks), assessment of separation (peak capacity), single and multiple objective optimization approach, advanced optimization algorithms (genetic algorithms, simulated annealing) and method transfer issues (transfer of methods between instruments and / or laboratories). Selected topics provide an accessible source of information needed for successful increase of chromatographic efficiency and economic feasibility (higher sample throughput) in liquid chromatography.

Keywords

liquid chromatography; method development; chemometrics

Hrčak ID:

126949

URI

https://hrcak.srce.hr/126949

Publication date:

1.7.2014.

Visits: 2.127 *