Preliminary communication
https://doi.org/10.15644/asc48/3/5
A Fast Method for DEFB1-44C/G SNP Genotyping in Brazilian Patients with Periodontitis
Renata Cimões
orcid.org/0000-0003-3673-8739
; PhD, Federal University of Pernambuco, Recife, Brazil
Rafael Rafael Amorim Cavalcanti de Siqueira
; PhD, Federal University of Pernambuco, Recife, Brazil
Sergio Crovella
; PhD, Federal University of Pernambuco, Recife, Brazil
Paulo Roberto Eleutério de Souza
; PhD, Federal University of Pernambuco, Recife, Brazil
Nikos Donos
; PhD, Eastman Dental Institute, Unit Periodontology, London, United Kingdom
Abstract
Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparison to healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in
distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigate the role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.
Keywords
Defensins; Antimicrobial Cationic Peptides; DEFB1 protein, human; Epithelial Cells; Diabetes Complications; Periodontitis
Hrčak ID:
127324
URI
Publication date:
25.9.2014.
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