Original scientific paper

Determination of Dazomet in Basamid Granulat Using Reversed Phase HPLC

Biljana R. Petanovska-Ilievska ; Laboratory for Pesticide Formulation Control, Department of Chemistry, Faculty of Agriculture, Bul. Aleksandar Makedonski bb, 91000 Skopje, P.O. Box 297, Republic of Macedonia
Lila B. Vodeb ; Laboratory for Pesticide Formulation Control, Department of Chemistry, Faculty of Agriculture, Bul. Aleksandar Makedonski bb, 91000 Skopje, P.O. Box 297, Republic of Macedonia

Abstract

The reverse phase HPLC determination of dazomet (tetrahydro-3,5-dimethyl-1,3,5-thiadiazine-2-tione) in a pesticide formulation has been studied. HS Pecosphere 3 x 3 C8 (3 μm, 3.3 x 0.46 cm) and LiChrosorb C8 (5 μm, 25 x 0.4 cm) analytical columns were tested at a flow rate of 1.0 cm3 min-1, column temperature of 25 °C and UV detection at 280 nm. The best separation of dazomet from its dehydro-dimer forms was achieved with a mobile phase containing acetonitrile-water in the volume ratio 15 : 85 on a HS Pecosphere column, and acetonitrile-water in the volume ratio 30 : 70 on a LiChrosorb C8 column. The HS Pecosphere column showed a better peak symmetry, separation factor, and multiple correlation coefficient. The retention time and peak area for the HS Pecosphere column were precise within a day and between days as indicated by the ANO VA test, in contrast to the LiChrosorb column, which showed lost of efficiency.

Keywords

reversed-phase HPLC; tetrahydro-3,5-dimethyl-1,3,5-thiadiazine-2-tione

Hrčak ID:

127498

URI

https://hrcak.srce.hr/127498

Publication date:

4.2.2002.

Visits: 1.502 *