Original scientific paper

Langmuir Aggregation of Dahliaviolett on DNA

Hong-Wen Gao ; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou-310014, P. R. China

Abstract

Formation of double electrostatic films in DNA causes Langmuir aggregation of small ions and molecules only in a monolayer. We studied the interaction of dahliaviolett (DLV) with DNA at pH = 7.24 by a spectral correction technique. Results showed that the ad-sorption ratio of DLV to DNA-P was 1 : 4, the adsorption constant of the DLV-DNA aggregate was 4.44 x 104 and its molar absorption coefficient was 1.02 x 105 dm3 mol-1 cm-1 at 615 nm. This aggregation was applied to the quantitative determination of DNA in samples.

Keywords

DNA; dahliaviolett; double electrostatic films; Langmuir aggregation; spectral correction technique

Hrčak ID:

127549

URI

https://hrcak.srce.hr/127549

Publication date:

1.8.2002.

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