Original scientific paper
https://doi.org/10.5599/jese.2014.0051
Analytical method for heavy metal determination in algae and turtle eggs from Guanahacabibes Protected Sea Park
Abel I. Balbín Tamayo
; Department of Food Science, University of Teramo, 64023, Teramo, Italy
Ana M Esteva Guas
; Department of Analytical Chemistry, Faculty of Chemistry, University of Havana, Havana, 10400, Cuba
Juan J. Piña Leyte-Vidal
; Department of Analytical Chemistry, Faculty of Chemistry, University of Havana, Havana, 10400, Cuba
Marcelo Maccini
; Department of Food Science, University of Teramo, 64023, Teramo, Italy
Abstract
A standard digestion method coupled to electrochemical detection for the monitoring of heavy metals in biological samples has been used for the simultaneous analysis of the target analytes. Square wave anodic stripping voltammetry (SWASV) coupled to disposable screen-printed electrodes (SPEs) was employed as a fast and sensitive electroanalytical method for the detection of heavy metals. The aim of our study was to determine Cd, Pb and Cu by SWASV in brown algae (Sargasum natan) and green turtle eggs (Chelonia mydas) using screen-printed electrodes. The method proved useful for the simultaneous analysis of these metals by comparison between two different procedures for preparing the samples. Two different approaches in digestion protocols were assessed. The study was focused on Guanahacabibes brown algae and green turtle eggs because the metal concentrations recorded in this area may be used for intraspecific comparison within the Guanahacabibes Protected Sea Park area, a body of water for which information is still very scarce. The best results were obtained by digesting biological samples with the EPA 3050B method. This treatment allowed the fast and quantitative extraction from brown algae and green turtle eggs of the target analytes, with high sensitivity and avoiding organic residues, eventually affecting electrochemical measurements.
Keywords
Cadmium; copper; lead; Sargasum Natan; Chelonia Mydas eggs; square-wave anodic stripping
Hrčak ID:
130206
URI
Publication date:
6.12.2014.
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