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Archives of Industrial Hygiene and Toxicology, Vol. 65 No. 4, 2014.

Original scientific paper

https://doi.org/10.2478/10004-1254-65-2014-2547

In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout

Ahmet Topal ; Department of Basic Sciences Faculty of Fisheries, Ataturk University Erzurum, Turkey
Muhammed Atamanalp ; Department of Aquaculture Ataturk University, Erzurum, Turkey
Ertan Oruç ; Faculty of Fisheries, Department of Pathology, Faculty of Veterinary, Ataturk University, Erzurum, Turkey
Yeliz Demir ; Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey
Şükrü Beydemir ; Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey
Alparslan Işık ; Department of Basic Sciences Faculty of Fisheries, Ataturk University Erzurum, Turkey

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Abstract

Chlorpyrifos is an organophosphate pesticide widely used in agriculture and aquaculture. This study investigated its effects on carbonic anhydrase (CA) enzyme activity and histopathology of rainbow trout gill and liver. The fish were exposed to 2.25 (25 % of 96 h LC50), 4.5 (50 % of 96 h LC50), and 6.75 μg L-1 (75 % of 96 h LC50) of chlorpyrifos for 24, 48, 72, and 96 h. CA activity was measured in liver and gills and histopathological changes were examined by light microscopy. The most common liver changes at most of the chlorpyrifos concentrations were hyperaemia and degenerative changes. Gill tissues were characterised by lamellar hyperaemia, lamellar oedemas, clumping, cellular degeneration, hyperplasia, and lamellar atrophy. CA enzyme activity in the gills decreased at all concentrations at 48, 72, and 96 h after exposure to chlorpyrifos (p<0.05). Similarly, there was a time-dependent decrease in CA activity at all of the concentrations in liver tissues (p<0.05). The present study indicated that chlorpyrifos inhibits CA enzyme activity and causes histopathological damage in gill and liver tissues.

Keywords

acute toxicity; fish; histology; light microscopy; organophosphate pesticides

Hrčak ID:

130345

URI

https://hrcak.srce.hr/130345

Publication date:

9.12.2014.

Article data in other languages: croatian

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