Original scientific paper

The hematological profile of farmed Sorubim lima: reference intervals, cell morphology and cytochemistry.

Micael B. Bianchi ; Aquatic Organisms Health Laboratory - AQUOS, Aquaculture Department, Federal University of Santa Catarina - UFSC, Admar Gonzaga highway, Florianópolis, SC, Brazil
Gabriela T. Jerônimo ; Aquatic Organisms Health Laboratory - AQUOS, Aquaculture Department, Federal University of Santa Catarina - UFSC, Admar Gonzaga highway, Florianópolis, SC, Brazil
Santiago B. Pádua ; Aquatic Organisms Health Laboratory - AQUOS, Aquaculture Department, Federal University of Santa Catarina - UFSC, Admar Gonzaga highway, Florianópolis, SC, Brazil
Fabiana Satake ; Aquatic Organisms Health Laboratory - AQUOS, Aquaculture Department, Federal University of Santa Catarina - UFSC, Admar Gonzaga highway, Florianópolis, SC, Brazil
Márcia M. Ishikawa ; Aquatic Organisms Health Laboratory - AQUOS, Aquaculture Department, Federal University of Santa Catarina - UFSC, Admar Gonzaga highway, Florianópolis, SC, Brazil
Marcos Tavares-Dias ; Aquatic Organisms Health Laboratory - AQUOS, Aquaculture Department, Federal University of Santa Catarina - UFSC, Admar Gonzaga highway, Florianópolis, SC, Brazil
Maurício L. Martins ; Aquatic Organisms Health Laboratory - AQUOS, Aquaculture Department, Federal University of Santa Catarina - UFSC, Admar Gonzaga highway, Florianópolis, SC, Brazil

Abstract

Hematology implies rapid and practical analysis to assist the diagnosis of fish homeostatic imbalance. This study determined the blood reference intervals in an important native South American catfish farmed in the Brazilian Pantanal wetland, Sorubim lima (Siluriformes, Pimelodidae), and describes the morphological and cytochemical characteristics of the cells. A total of 92 fish reared in a fish farm were examined for reference intervals 25 - 75% of total plasmatic protein, hemoglobin, red blood cell count (RBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) and total thrombocyte and leukocyte count (WBC). Periodic acid Schiff (PAS) with salivary amylase, bromophenol blue, Sudan black B and toluidine blue (metacromasy) were the cytochemical stains employed to signal glycogen, proteins, lipids and nuclei in thrombocytes and leucocytes. In the blood smears, monocytes, lymphocytes, neutrophils, basophils, eosinophils, PAS - positive granular lymphocytes (LG-PAS) and thrombocytes were observed. Siluriforme similar results were observed in neutrophils and eosinophils morphology. Compared to other pimelodidae RBC, hematocrit and MCV showed the lowest index variation. In contrast to other siluriformes fish, S. lima showed the concomitant presence of circulating eosinophils and basophils.

Keywords

freshwater fish; jurupensém; health; hematology; farming

Hrčak ID:

130431

URI

https://hrcak.srce.hr/130431

Publication date:

5.12.2014.

Article data in other languages: croatian

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