Original scientific paper
Genotoxic Potential of Dentin Bonding Agents
Dunja Prica
Nada Galić
Davor Želježić
Adriana Prica
Sanja Šegović
Abstract
The aim of this study was to examine the genotoxic activity of five dentin bonding agents: Adper Single Bond, Adper Single Bond 2, Prompt L-pop, Excite and Opti-Bond Solo Plus. This in vitro study was performed on human lymphocytes from peripheral blood, and the concentrations of dentin bonding agents tested were 0.2, 0.5 i 5 μg/ml, and elution times tested were 1 hour, 24 hours and 5 days. Genotoxicity testing was done using the Structural chromosomal aberration analysis cytogenic method , which determined the complete number of chromosome breaks, chromatide breaks and acentric fragments. The results showed genotoxicity of Opti-Bond Solo Plus in the 0.2 μg/ml concentration after a 24-hour elution period, then OptiBond Solo Plus in the 0.5 μg/ml concentration after a 1 hour elution period and OptiBond Solo Plus, Adper Single Bond 2 and Excite in the 0.5 μg/ml concentration after a 1 day elution period. In the 5 μg/ml concentration after 1 hour of elution, genotoxic potential was observed in cultures with OptiBond Solo Plus, Excite, Adper Single Bond 2 and Adper Single Bond, while all dentin bonding agents showed genotoxicity at that highest concentration but after 1 day of elution. From the results it is obvious that genotoxicity increases with the concentration of the dentin bonding agent, and decreases over time. The highest genotoxicity was observed after a 24-hour-elution period.
Keywords
Mutagens; Dentin-Bonding Agents; Chromosome Aberrations
Hrčak ID:
12541
URI
Publication date:
15.6.2007.
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