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Original scientific paper

Association of Interleukin 10 And Transforming Growth Factor β Gene Polymorphisms with Chronic Idiopathic Urticaria

Marzieh Tavakol
Masoud Movahedi
Ali Akbar Amirzargar
Zahra Aryan
Alireza Zare Bidoki
Kimia Heidari
Samaneh Soltani
Mohammad Gharagozlou
Asghar Aghamohammadi
Mohammad Nabavi
Rasoul Nasiri
Alireza Ahmadvand
Nima Rezaei


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Abstract

Transforming growth factor β (TGF-β) and interleukin 10 (IL-10) are two anti-inflammatory cytokines that are implicated in the pathogenesis of urticaria. The goal of this study was to examine the possible association of polymorphisms of TGF-β and IL-10 genes with susceptibility to chronic idiopathic urticaria (CIU). This study was conducted on 90 patients with CIU. Polymerase chain reaction (PCR) was done to determine the genotype at 5 polymorphic sites; TGF-β (codon10C/T and codon25G/C) and IL-10 (-1082G/A, -819C/T, and -592C/A). The C allele at codon 25 of TGF-β was more prevalent in CIU patients compared to controls (OR = 9.5, 95% CI = 5.4-16.8, P<0.001). Genotypes of CT and CG at 10 and 25 codons of TGF-β gene, respectively, and AG, CT, and CA for loci of -1082, -819, and -592 of IL-10 gene were significantly higher in CIU patients (P<0.001). In haplotype analysis, frequency of TGF-β haplotypes differed between patients with CIU and controls; CC haplotype was overrepresented, while CG and TG haplotypes were underrepresented (P<0.001). These results suggest that TGF-β and IL-10 genetic variability could contribute to susceptibility to CIU. Additionally, patients with CIU seem to have genotypes leading to high production of TGF-β and IL-10.

Keywords

Hrčak ID:

130862

URI

https://hrcak.srce.hr/130862

Publication date:

17.12.2014.

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