Croatica Chemica Acta, Vol. 87 No. 4, 2014.
Review article
https://doi.org/10.5562/cca2488
Intrinsic Properties of Nitric Oxide Binding to Ferrous and Ferric Hemes
Barbara Chiavarino
; Dipartimento di Chimica e Tecnologie del Farmaco, Università di Roma “La Sapienza”, P. le A. Moro 5, I-00185, Roma, Italy
Maria Elisa Crestoni
; Dipartimento di Chimica e Tecnologie del Farmaco, Università di Roma “La Sapienza”, P. le A. Moro 5, I-00185, Roma, Italy
Simonetta Fornarini
; Dipartimento di Chimica e Tecnologie del Farmaco, Università di Roma “La Sapienza”, P. le A. Moro 5, I-00185, Roma, Italy
Abstract
Gas phase studies offer an ideal medium whereby structural and reactivity properties of charged spe-cies may be unveiled in the absence of solvent, matrix or counterion effects. In this environment NO binds to iron(II)- and iron(III)-hemes with comparable kinetics and equilibrium parameters, conclu-sively elucidating the factors determining the widely different affinity in protic solvents or in heme proteins. IRMPD spectroscopy of the isolated species provides unambiguous characterization of the gaseous
nitrosyl heme complexes.
Keywords
five-coordinate hemes; nitrosyl complexes; FT-ICR mass spectrometry; IR spectroscopy; NO addition kinetics; metallobiomolecules
Hrčak ID:
131522
URI
Publication date:
22.12.2014.
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