Original scientific paper

Large-scale Solution Synthesis of Phosphorothioate Oligonucleotides: A Comparison of the Phosphoro-amidite and Phosphotriester Dimeric Approaches

Gian Maria Bonora ; Department of Chemical Sciences, University of Trieste, Via Giorgieri 1, Trieste, Italy
Simone Zaramella ; MBB, Karolinska Institutet, Stockolm, Sweden
Vasulinga Ravikumar ; ISIS Pharmaceutical, Carlshad, California, USA

Abstract

Two different approaches to the solution synthesis of oligonucleotides are compared. Both procedures use dimer synthons as building blocks to save time and to improve the final product yield and quality. These strategies look quite promising due to their easy up-scaling, providing an advantageous alternative to the well-established solid-phase methods.

Keywords

oligonucleotides; phosphorothioate; scale up; large-scale; synthesis; phosphoramidite; phosphotriester; liquid-phase; polyethylene glycol

Hrčak ID:

131954

URI

https://hrcak.srce.hr/131954

Publication date:

1.11.2001.

Visits: 1.454 *