Original scientific paper

Flavonoids of Guiera senegalensis J. F. GMEL. − Thin-layer Chromatography and Numerical Methods

Željan Maleš ; Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Marica Medić-Šarić ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Franz Bucar ; Institute of Pharmacognosy, Karl-Franzens-University, Graz, Austria

Abstract

The efficiency of seven thin-layer chromatographic systems for separating nine flavonoids isolated from Guiera senegalensis J. F. GMEL. (Combretaceae) was investigated. For this purpose, three mathematical techniques were applied. The first technique was the calculation of the information content derived from Shannon’s equation for each chromatographic system. In the second technique, the discriminating power of the systems was measured individually and in various combinations. The third technique classifies chromatographic systems according to clusters. The classification was carried out by the numerical taxonomy methods. The most suitable chromatographic systems for the separation of the investigated flavonoids are: ethyl acetate : ethylmethylketon : formic acid : water, 60:15:3:2, and ethyl acetate : formic acid : acetic acid : water, 100:11:11:27.

Keywords

Hrčak ID:

132325

URI

https://hrcak.srce.hr/132325

Publication date:

2.3.1998.

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