Original scientific paper
HIV subtype diversity and primary resistance to antiretroviral drugs in newly diagnosed HIV-infected persons from Croatia in 2013
Ivana Grgić
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Ana Planinić
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Lana Gorenec
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Adriana Vince
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Josip Begovac
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Snježana Židovec Lepej
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Abstract
The aim of this study was to analyse HIV subtype diversity as well as primary resistance to antiretroviral drugs in 30 treatment-naive individuals entering clinical care at the National Refence Center for HIV/AIDS during 2013. Sequencing the pol region of the viral genome and bioinformatic algorithms were used to determine HIV subtypes and to analyse transmitted drug resistance mutations. The majority of individuals (86,7 %) were infected with HIV-1 subtype B. Twelve mutations associated with primary resistance to antiretroviral drugs were detected in the study: 9 mutations conferring resistance to NRTI (nucleotide reverse-transcriptase inhibitors) and 3 mutations associated with resistance to NNRTI (non-nucleotide reverse-transcriptase inhibitors). Primary resistance to two antiretroviral drug classes (NRTI and NNRTI) were detected in one individual. The most frequent mutation was T1215S associated with primary resistance to NRTI. Drug resistance mutations were detected in 4 subtype B and 2 non-B HIV-infected individuals. This study showed, for the first time, the presence of mutations associated with primary resistance of HIV to antiretroviral drugs in individuals infected with non-B subtypes in Croatia.
Keywords
HIV; subtypes; primary resistance; mutations
Hrčak ID:
133440
URI
Publication date:
31.12.2013.
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