Croatica Chemica Acta, Vol. 70 No. 1, 1997.
Original scientific paper
Electrochemical Characterization of Metal-Binding Properties of Metallothioneins Isolated from M. galloprovincialis
Biserka Raspor
; Rudjer Bošković Institute, Center for Marine Research, P.O.B. 1016, 10001 Zagreb, Croatia
Jasenka Pavičić
; Rudjer Bošković Institute, Center for Marine Research, P.O.B. 1016, 10001 Zagreb, Croatia
Abstract
Amperometric titrations of defined metallothionein concentrations with the standard CdCl2 solution have been performed in seawater samples at pH << 1 and pH >> 8, in order to define the metal-binding properties of purified mussel metallothionein component. The concentration of the formed Cd-thionein complex has been assessed in the indirect and direct mode. The results on the available ligand concentration CL for complexing Cd2+ ions and the apparent stability constant K, determined by two procedures are compared and the differences discussed. Complexing characteristics of the metallothionein can be reliably determined only if the evaluation is based on the specific signal of Cd-thionein complex.
Keywords
Hrčak ID:
135618
URI
Publication date:
3.4.1997.
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