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Journal of Electrochemical Science and Engineering, Vol. 5 No. 1, 2015.

Short communication, Note

https://doi.org/10.5599/jese.2015.0072

Electrochemical immunosensor for the determination of β-casein

Judith Molinari ; Unidad Técnica Nanomateriales, INTI-Procesos Superficiales, Instituto Nacional de Tecnología Industrial, C.C. 157, B1650WAB San Martín, Argentina
Carlos Moina ; Unidad Técnica Nanomateriales, INTI-Procesos Superficiales, Instituto Nacional de Tecnología Industrial, C.C. 157, B1650WAB San Martín, Argentina
Gabriel Ybarra ; Unidad Técnica Nanomateriales, INTI-Procesos Superficiales, Instituto Nacional de Tecnología Industrial, C.C. 157, B1650WAB San Martín, Argentina

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Abstract

An amperometric biosensor for the quantification of food allergens based on an inhibitory immunoassay is presented. As a proof of concept, the experimental conditions were optimized for the detection of β-casein in the 0-10 ppm range. Eight electrochemi-cal cells were integrated into a small-sized portable potentiostat controlled by a smart-phone via Bluetooth communication. The determination of β-casein in eight different samples can be measured with the electrochemical biosensor, which has the potential to be modified for the detection of multiple allergens.

Keywords

Allergen; Casein; Electrochemistry; ELISA; Immunoassay

Hrčak ID:

136651

URI

https://hrcak.srce.hr/136651

Publication date:

15.3.2015.

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