Original scientific paper

Physiological Characteristics of Some Monoamine Metabolites in Cat Cerebrospinal Fluid

Darko Orešković ; Department of Chemistry, »Ruđer Bošković« Institute, Bijenička 54, HR-10 000 Zagreb, Croatia
Mauricio Sanković ; Department of Chemistry, »Ruđer Bošković« Institute, Bijenička 54, HR-10 000 Zagreb, Croatia
Ana Fröbea ; Department of Chemistry, »Ruđer Bošković« Institute, Bijenička 54, HR-10 000 Zagreb, Croatia
Marijan Klarica ; Department of Pharmacology, Medical Faculty University of Zagreb, Šalata 11, HR-10 000 Zagreb, Croatia

Abstract

The concentrations of main metabolites of serotonin and dopamine, 5-hydroxyindoleacetic acid and homovanillic acid, respectively, were measured in cisternal cerebrospinal fluid of cats by high performance liquid chromatography with an electrochemical detector.
Higher concentrations of homovanillic acid and a wide interindividual oscillation for both parameters have been found. However, samples collected at four different time intervals showed stabile intraindividual concentrations of the metabolites.
The existence of a concentration gradient of both parameters inside the cat cerebrospinal fluid system was confirmed in experiments with a provoked artificial flow of cerebrospinal fluid inside physiological limits. These experiments also suggested that the hydrodynamics of cerebrospinal fluid is the factor responsible for the concentration gradient existence. It appears that the absence of cerebrospinal fluid circulation is the main hydrodynamic reason for the maintenance of 5-hydroxyindoleacetic acid and homovanillic add gradients between various parts of the cerebrospinal fluid system.

Keywords

Hrčak ID:

136711

URI

https://hrcak.srce.hr/136711

Publication date:

1.9.1995.

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