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Conference paper

Interaction of Linear Plasmid with Streptomyces rimosus Chromosome: Evidence for the Linearity of Chromosomal DNA

Daslav Hranueli ; PLIVA d.d., Research Institute, HR-10 000 Zagreb, Republic of Croatia
Kenan Pandža ; LB Genetik, Universitát Kaiserslautern, D-67653 Kaiserslautern, F.R.G.
Goran Biuković ; PLIVA d.d., Research Institute, HR-10 000 Zagreb, Republic of Croatia
Birgit Gravius ; LB Genetik, Universitát Kaiserslautern, D-67653 Kaiserslautern, F.R.G.
John Cullum ; LB Genetik, Universitát Kaiserslautern, D-67653 Kaiserslautern, F.R.G.


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Abstract

Recent studies imply that a linear chromosome may be characteristic of the Streptomyces genus. S. rimosus R6, the oxytetracy- cline (OTC) producer, carries a linear plasmid pPZGIOl that can interact with the bacterial chromosome to form integrants and plasmid-primes. Restriction analysis of one integrant (MV25) showed that pPZGIOl sequence had been integrated into the chromosome together with its linear end(s). Another strain (MV17) carried a linear plasmid of increased size of about 1 Mb (pPZG103).
It was shown that OTC-genes were carried on the pPZG103, indicating that the plasmid was pPZGIOl'-OTC. Restriction mapping of pPZG103 demonstrated that one plasmid end was derived from the parental pPZGIOl while the other end was a linear chromosomal end. The linearity of S. rimosus R6 chromosomal DNA was further supported by the development of the chromosomal physical map.

Keywords

Hrčak ID:

136717

URI

https://hrcak.srce.hr/136717

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