Conference paper

Structure of the Saccharomyces cerevisiae Cell Wall

Renata Vuković ; Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10 000 Zagreb, Croatia
Vladimir Mrša ; Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10 000 Zagreb, Croatia

Abstract

Cell wall of the yeast S. cerevisiae is composed of the structural polysaccharides glucan, mannan and chitin, and of proteins. Possible interactions between the wall components have not yet been studied in detail. Molecular organization of the cell wall constituent molecules is also, to a great extent, unclear, lb elucidate the structure of the cellular outermost compartment, intermolecular interactions responsible for the constitution of the cell wall were studied. Results revealed protein-glucan, protein-chitin, glucan-chitin, and glucan-glucan interactions. Mannan chains reacted neither with proteins nor with other polysaccharides. Intact cell walls were found to bind proteins approaching the wall from the inside but not those reaching the cell wall from the outer side, suggesting a marked asymmetry of the wall having an outer mannan layer, and an inner glucan network. Mannoproteins seem to be attached to glucan through their protein moieties.

Keywords

Hrčak ID:

136719

URI

https://hrcak.srce.hr/136719

Publication date:

1.9.1995.

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