Original scientific paper
https://doi.org/10.15644/asc49/1/3
A Quantitative Analysis of a Probiotic Storage Media for Avulsed Teeth
Esber Caglar
; Private Practice, Istanbul, Turkey; Department of Pediatric Dentistry, Dental School, Yeditepe University, Istanbul, Turkey
Sertac Peker
; Department of Pediatric Dentistry, Dental School, Marmara University, Istanbul, Turkey
Muyesser Ahu Durhan
; Department of Pediatric Dentistry, Dental School, Marmara University, Istanbul, Turkey
Pinar Kulan
; Department of Pediatric Dentistry, Dental School, Marmara University, Istanbul, Turkey
Ozgur Önder Kuscu
; Private Practice, Istanbul, Turkey; Department of Pediatric Dentistry, Dental School, Yeditepe University, Istanbul, Turkey
Rabia Pisiriciler
; Department of Histology, Dental School, Marmara University, Istanbul, Turkey
Caliskan Enis Ak
; Department of Histology, Dental School, Marmara University, Istanbul, Turkey
Betul Kargul
; Department of Pediatric Dentistry, Dental School, Marmara University, Istanbul, Turkey
Nuket Sandalli
; Department of Pediatric Dentistry, Dental School, Yeditepe University, Istanbul, Turkey
Abstract
Aim: The aim of the present in vitro study was to investigate the potential of a storage medium, probiotic yogurt (Bifidibacterium animalis DN 173010) in comparison with Hank’s balanced salt solution (HBSS), saline and milk in maintaining viable periodontal ligament (PDL) cells on simulated avulsed teeth. Materials and methods: Thirty-six freshly extracted single-rooted human teeth with closed apices were divided into six experimental groups (N=6). The teeth were extracted as atraumatically as possible and washed in sterile saline solution to eliminate residual blood. Following extractions, the coronal 3 mm of PDL tissues were scraped with a #15 scalpel to remove cells that may have been damaged. The positive and negative controls corresponded to 0 minutes and an 8-hour dry time, respectively. After extraction, the positive control teeth were immediately treated with dispase and collagenase. The negative control teeth were bench-dried for 8 h, with no follow-up storage solution time, and then placed in the dispase and collagenase. The number of viable protective least significant difference PDL cells were counted under a light microscope
with a hemocytometer at 20x magnification and analyzed. Statistical analysis of the data was accomplished using Nonparametric ANOVA complemented by Kruskal-Wallis Test and Dunn’s Multiple Comparisons Test. Results: Positive control was found to be significantly better than the others, there were statistically significant differences between positive control and other test groups (p=0.000). The teeth stored in positive control demonstrated the highest number of viable PDL cells followed in order by probiotic yogurt, HBSS, saline and milk. Conclusion: Bifidibacterium animalis DN 173010 seems to be an alternative for the temporary storage of avulsed teeth, due to high number of viable PDL cells. Probiotics may be suitable transport media for avulsed teeth, but further research is warranted using the commercially available products.
Keywords
Tooth Avulsion; Tissue Preservation; Periodontal Ligament; Milk; Hanks Balanced Salt Solution; Saliva; Probiotics
Hrčak ID:
136814
URI
Publication date:
24.3.2015.
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