Original scientific paper

Sulphated Polysaccharides of Brown Seaweeds Cystosira compressa, Fucus virsoides, and Dyctiopteris membranacea

Ante Pelivan ; Department of Chemistry and Biochemistry, Veterinary Faculty, University of Zagreb, 41000 Zagreb, Croatia
Aleksandar Lutkić ; Department of Chemistry and Biochemistry, Veterinary Faculty, University of Zagreb, 41000 Zagreb, Croatia

Abstract

Sulphated polysaccharides have been isolated from three kinds of brown algae - Fucus virsoides, Cystosira compressa, and Dyctiopteris membranacea. The specimens of the algae were collected during three different seasons. Extraction of sulphated polysaccharides was carried out using different media and at different temperatures. Extractive fractions were precipitated by successive adding of ethanol containing magnesium chloride. The purity of single fractions was checked by electrophoresis on cellulose acetate. Following total acid hydrolysis, the composition of each single fraction was studied by gas chromatography. Xylose, manose, galactose and a-L-fucose were identified in various proportions. Low concentrations of glucose were identified in a smaller number of fractions. Uronic acids, sulphate, and proteins occur in quite variable amounts. So, the investigated algae synthesize a wide range of sulphated polysaccharides which can be separated from the complex mixture. Seasonal variation in the composition of the fractions are of minor importance.

Keywords

Hrčak ID:

136882

URI

https://hrcak.srce.hr/136882

Publication date:

1.11.1994.

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