Original scientific paper
https://doi.org/10.14798/73.1.776
GENETIC DIVERSITY OF OLIVE BARB (Systomus sarana, Hamilton, 1822) FROM DIFFERENT LOCATIONS OF BANGLADESH
Md. Alamgir Kabir
; Department of Fisheries, University of Dhaka, Dhaka 1000, Bangladesh
Md. Ahashan Habib
; Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh
Anwar Hossain
; Department of Fisheries, University of Dhaka, Dhaka 1000, Bangladesh
Shankar Chandra Mandal
orcid.org/0000-0002-0466-4440
; Department of Fisheries, University of Dhaka, Dhaka 1000, Bangladesh
Abstract
Olive barb (Systomus sarana) was collected from three different locations viz. Kuliarchar, Bhairab and Bikrampur in Bangladesh to elucidate genetic diversity by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). Six arbitrary oligonucleotide RAPD primers were used to amplify the DNA from each population. A total of 52 bands were produced in 3 olive barb populations where 28 bands were polymorphic indicating 53.84% polymorphisms in those three populations with an average of 9 bands per primer. The molecular size of the amplified DNA fragments
ranged between 300 and 2300 bp. 12 unique RAPD bands were observed in the three populations. The bands are specific and stable and thus could be used to characterize each germplasm. The values of pair-wise genetic distances ranged between 0.5232 and 0.8109 with some degrees of genetic variation among the populations. The highest genetic distance (0.8109) was observed between olive barb collected from Kuliarchar and Bikrampur, while the lowest (0.5232) was found between olive barb collected from Kuliarchar and Bhairab. The UPGMA dendrogram segregated three samples of olive barb into two major clusters C1 and C2. The Kuliarchar-Bhairab population pair of olive barb was genetically closer than that of Kuliarchar-
Bikrampur. Therefore, RAPD analysis can be used to identify the genetic diversity in olive barb. This information may be used for improved breeding programme and conservation of native olive barb.
Keywords
RAPD-PCR technique; Genomic DNA; DNA fingerprinting; Genetic diversity
Hrčak ID:
137587
URI
Publication date:
25.3.2015.
Visits: 1.774 *