Original scientific paper
https://doi.org/10.15177/seefor.14-18
Mycorrhizal Fungal Community of Poplars Growing on Pyrite Tailings Contaminated Site near the River Timok
Marina Katanić
orcid.org/0000-0003-2993-1637
; Institute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, RS-21000 Novi Sad, Serbia
Saša Orlović
; Institute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, RS-21000 Novi Sad, Serbia
Tine Grebenc
; Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia
Branislav Kovačević
; Institute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, RS-21000 Novi Sad, Serbia
Marko Kebert
; Institute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, RS-21000 Novi Sad, Serbia
Milan Matavulj
; European University, Faculty of Pharmacy, Trg mladenaca 5, RS-21000 Novi Sad, Serbia
Hojka Kraigher
; Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia
Abstract
Background and Purpose: Mycorrhizal fungi are of high importance for functioning of forest ecosystems and they could be used as indicators of environmental stress. The aim of this research was to analyze ectomycorrhizal community structure and to determine root colonization rate with ectomycorrhizal, arbuscular mycorrhizal and endophytic fungi of poplars growing on pyrite tailings contaminated site near the river Timok (Eastern Serbia).
Materials and Methods: Identification of ectomycorrhizal types was performed by combining morphological and anatomical characterization of ectomycorrhizae with molecular identification approach, based on sequencing of the nuclear ITS rRNA region. Also, colonization of poplar roots with ectomycorrhizal, arbuscular mycorrhizal and dark septated endophytic fungi were analysed with intersection method.
Results and Conclusions: Physico-chemical analyses of soil from studied site showed unfavourable water properties of soil, relatively low pH and high content of heavy metals (copper and zinc). In investigated samples only four different ectomycorrhizal fungi were found. To the species level were identified Thelephora terrestris and Tomentella ellisi, while two types remained unidentified. Type Thelephora terrestris made up 89% of all ectomycorrhizal roots on studied site. Consequently total values of Species richness index and Shannon-Weaver diversity index were 0.80 and 0.43, respectively. No structures of arbuscular mycorrhizal fungi were recorded. Unfavourable environmental conditions prevailing on investigated site caused decrease of ectomycorrhizal types diversity. Our findings point out that mycorrhyzal fungal community could be used as an appropriate indicator of environmental changes.
Keywords
ctomycorrhiza; molecular identification; poplars; Timok; pyrite tailing
Hrčak ID:
139168
URI
Publication date:
19.5.2015.
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