Original scientific paper

Changes in nutrition type between generations influence on bone structural changes in rat female offspring

ANĐELA MARIĆ ; 1_Department of Anatomy and Neuroscience, Faculty of Medicine, University of Osijek, Osijek, Croatia; 2_Department of Physical Medicine and Rehabilitation, University Hospital Centre Osijek, Bizovac, Croatia
ŽELJKA PERIĆ KAČAREVIĆ ; Department of Anatomy and Neuroscience, Faculty of Medicine, University of Osijek, Osijek, Croatia
ROBERT SELTHOFER ; 1_Department of Anatomy and Neuroscience, Faculty of Medicine, University of Osijek, Osijek, Croatia; 2_Department of Obstetrics and Gynecology, University Hospital Centre Osijek, Osijek, Croatia

Abstract

Background and Purpose: It is believed that changes in feeding protocol
between generations have influence on the balance of the whole organism. Epidemiological studies suggest that skeletal growth is programmed during intrauterine and early postnatal life. The aim of the study was to determinate impact of maternal dietary fat excess and nutrition of female offspring on the bone structural changes in latter.

Materials and Methods: Ten female Sprague Dawley rats were randomly
divided in two groups. One group was fed with high content of saturated
fatty acid food (HFD) and the other with standard laboratory chow
(CD). Offspring from both groups were randomly divided in two subgroups
after coupling and lactation period, subsequently there were four groups of offspring (n=6 each) with different feeding protocol: a) CD-CD – control diet mothers and offspring, b) CD-HFD – control diet mothers and high fat diet offspring, c) HFD-CD – high fat diet mothers and control diet offspring and d) HFD-HFD – high fat diet mothers and offspring. At the age of 18 weeks in female offspring bone microstructure was analyzed in fifth lumbar vertebra using digital photographic images.

Results and Conclusions: The control diet female offspring of high fat
fed mothers showed the highest values of trabecular thickness and trabecular number, while the CD-HFD offspring group had the highest values of trabecular separation and cortical thickness. Maternal nutritional status affects the future development of offspring.

Keywords

Hrčak ID:

139524

URI

https://hrcak.srce.hr/139524

Publication date:

31.3.2015.

Visits: 1.334 *