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Archives of Industrial Hygiene and Toxicology, Vol. 66 No. 2, 2015.

Original scientific paper

https://doi.org/10.1515/aiht-2015-66-2655

Polymorphisms in DNA repair genes: link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation

Mirta Milić orcid id orcid.org/0000-0002-9837-7185 ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ružica Rozgaj ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Vilena Kašuba ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ana-Marija Jazbec orcid id orcid.org/0000-0003-2888-8518 ; Faculty of Forestry, University of Zagreb, Zagreb, Croatia
Boris Starčević ; University Hospital Dubrava, Department of invasive cardiology, Zagreb, Croatia
Barnaba Lyzbicki orcid id orcid.org/0000-0001-9509-8503 ; Department of Pharmacy and Biotechnology,University of Bologna, Bologna, Italy
Gloria Ravegnini ; Department of Pharmacy and Biotechnology,University of Bologna, Bologna, Italy
Corrado Zenesini ; Department of Public Health, Epidemiological Service, Local Health Authority of Bologna, Bologna, Italy
Muriel Musti ; Department of Public Health, Epidemiological Service, Local Health Authority of Bologna, Bologna, Italy
Patrizia Hrelia ; Department of Pharmacy and Biotechnology,University of Bologna, Bologna, Italy
Sabrina Angelini ; Department of Pharmacy and Biotechnology,University of Bologna, Bologna, Italy

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Abstract

Individual sensitivity to ionising radiation (IR) is the result of interaction between exposure, DNA damage, and its repair, which is why polymorphisms in DNA repair genes could play an important role. We examined the association between DNA damage, expressed as micronuclei (MNi), nuclear buds (NBs), and nucleoplasmic bridges (NPBs) and single nucleotide polymorphisms in selected DNA repair genes (APE1, hOGG1, XRCC1, XRCC3, XPD, PARP1, MGMT genes; representative of the different DNA repair pathways operating in mammals) in 77 hospital workers chronically exposed to low doses of IR, and 70 matched controls. A significantly higher MNi frequency was found in the exposed group (16.2±10.4 vs. 11.5±9.4; P=0.003) and the effect appeared to be independent from the principal confounding factor. Exposed individuals with hOGG1, XRCC1, PARP1, and MGMT wild-type alleles or APEX1, as well as XPD (rs13181) heterozygous showed a significantly higher MNi frequency than controls with the same genotypes. Genetic polymorphism analysis and cytogenetic dosimetry have proven to be a powerful tool complementary to physical dosimetry in regular health surveillance programmes.

Keywords

genotype analysis; micronucleus; nuclear bud; nucleoplasmic bridge; occupational exposure

Hrčak ID:

139883

URI

https://hrcak.srce.hr/139883

Publication date:

16.6.2015.

Article data in other languages: croatian

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