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METHODS FOR DETECTION OF ANTIBODIES TO HLA ANTIGENS IN PATIENTS AWAITING KIDNEY TRANSPLANTATION AND COMBINED PANCREAS-KIDNEY TRANSPLANTATION

MIRELA ANČIĆ orcid id orcid.org/0000-0002-9854-2611 ; Split University Hospital Center, Department of Transfusion Medicine, Split, Croatia
BLAŽENKO MARAVIĆ ; Split University Hospital Center, Department of Urology, Split, Croatia
MAJA RADMAN ; Split University Hospital Center, University Department of Internal Medicine, Split, Croatia
VEDRAN KOVAČIĆ ; Split University Hospital Center, University Department of Internal Medicine, Split, Croatia


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Abstract

The best possibility to treat chronic renal disease is renal transplantation. Especially important fact in transplantation is the
percentage of so-called panel reactive antibody (PRA) that is focused on the human leukocyte antigen. There are several methods to determine the percentage of PRA in sensitized patients awaiting kidney transplants. The most important is the complementdependent cytotoxicity. A higher value of PRA implies greater likelihood of positive cross-match with random donor and lower probability of receiving a transplant. Comparing the sensitivity of laboratory tests for determination of PRA percentage in patient serum, it is concluded that ELISA and fl ow cytometry proved to be more sensitive and specific.

Keywords

HLA antigens; kidney transplantation; combined pancreas-kidney transplantation

Hrčak ID:

142206

URI

https://hrcak.srce.hr/142206

Publication date:

22.7.2015.

Article data in other languages: croatian

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