Short communication, Note
Measurement of the distribution of m-xylene in rat tissues by head space gas chromatography
Prem Kumarathasan
; Environmental Health Centre, Room 332, PL 0803C, Tunne/s Pasture, Ottawa (Ont) K1A 0L2, Canada
Rein Otson
Ih Chu
Abstract
An automated head space-gas chromatography (HS-GC) method was developed and evaluated for reliability in measurement of m-xylene in rat tissues. For tissue samples spiked with m-xylene (n=2), the analytical precision was better than 12% relative standard deviation (RSD) over the concentration range of 0.1 to cca 100 µg/g for liver and kidney, 0.1 to 170 µg/g for brain, 1.2 to 250 µg/g for fat, and 0.006 to 50 µg/mL for blood. For rats sacrificed immediately after an acute exposure to 1100 ppm of m-xylene, the relative tissue m-xylene concentrations were in the ascending order as follows: brain ≤ blood ≤kidney ≤ liver « fat. A precision of < 13% RSD was generally obtained for duplicale tissue samples from exposed animals, with m-xylene concentrations of about 10 µg/g of tissue.
Keywords
automated HS-GC method; blood; distribution; inhalation exposure; m-xylene; tissue
Hrčak ID:
144267
URI
Publication date:
3.7.1998.
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