Review article

The comet assay: a new approach in genotoxicological research

Aleksandra Fučić ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska

Abstract

Trie comet assay is a last, simple and sensitive genoloxicologlcal technique for measuring DNA damage in an individual cell of virtually any cell type of animal or plant origin. Electrophoresis of complete cell genome (assuming a comet-like shape) combined with the image analysis systems for comet analysis provide densitometric and geometric parameters describing the complete comet as well as the head and tall. The comet optical density values are used to quantify the total comet fluorescence and hence indicate DNA content and the level ot damage. The application or this method in different fields makes it a power ful tool in human genotoxic study as well as in the estimation of environmental pollution.

Keywords

cytogenetics; electrophoresis; genotoxicology

Hrčak ID:

144332

URI

https://hrcak.srce.hr/144332

Publication date:

3.7.1998.

Article data in other languages: croatian

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